Closed DomenicoSkyWalker89 closed 4 years ago
@DomenicoSkyWalker89
You need to give a complete description. I wouldn't expect contrasts (0,1) and (1,0) to give similar results, because they are testing different parameters of the model. And, (-1, 1) is again testing something different. I would suggest having a read through perhaps the limma or edgeR user's guide (e.g., the case studies) to get a handle on how to pair design matrices and contrasts. Or, a general resource on linear modeling.
If you ask a question here, please give full details, e.g., what is the design matrix?
Sorry for the few information. I followed the CATALYST workflow using flow cytometry data.
Hi @lmweber , thanks for the pakage and the support. I have a doubt regarding the contrast. I have two groups (CTRL and TREATED), using contrast (0,1) all p value show no significance but changing contrast (1,0) five population are different. I also find this last result using the contrast (-1,1). This make me confused.
Thanks, Domenico