lpantano / isomiRs

analyze isomiRs from seqbuster tool
http://lpantano.github.io/isomiRs/
MIT License
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PLS-DA plot interpretation #14

Open palbioinfor opened 6 years ago

palbioinfor commented 6 years ago

Hi, It might sound a very basic question but I am little confused with my PLS-DA analysis in isomiRs. Can you please interpret this plot please? I am aware that it shows separation of the data but I am just wondering why one right upper corner is blank ? But, I think I would like someone to interpret this plot for me where I am trying to see the difference in two age groups. screen shot 2018-10-26 at 1 11 41 pm

lpantano commented 6 years ago

Hi,

Probably there is only two because is using two components only. This is similar to a PCA. The plot will show you the samples using each of the significant components, and then the distribution of samples in each component. T1 and T2 seem to have a different distribution for each group.

It seems there is a slight separation. You can get the topmost important features looking into the VIP element of the returned object. Let me know if you have any other questions

Cheers

On Fri, Oct 26, 2018 at 1:06 PM palbioinfor notifications@github.com wrote:

Hi, It might sound a very basic question but I am little confused with my PLS-DA analysis in isomiRs. Can you please interpret this plot please? I am aware that it shows separation of the data but I am just wondering why one right upper corner is blank ? But, I think I would like someone to interpret this plot for me where I am trying to see the difference in two age groups.

[image: screen shot 2018-10-26 at 1 02 18 pm] https://user-images.githubusercontent.com/36253759/47581607-dbd23480-d91f-11e8-9f2e-86a05a314b44.png

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