During alignment, it would be useful to have a quantitative measure of the spot size.
Perhaps even display a 1D intensity histogram (a slice of the image through centroid), so that the user can easily get a sense of the shape of the spot and background level.
During alignment, it would be useful to have a quantitative measure of the spot size. Perhaps even display a 1D intensity histogram (a slice of the image through centroid), so that the user can easily get a sense of the shape of the spot and background level.