Closed steve-shan closed 7 years ago
Which tool are you using CRISPResso or CRISPRessoPooled?
Crisprpooled
On Dec 2, 2016, at 3:21 PM, Luca Pinello notifications@github.com wrote:
Which tool are you using CRISPResso or CRISPRessoPooled?
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If you share the text file I can take a look.
Hello, This is the txt file.
On Dec 2, 2016, at 17:23, Luca Pinello notifications@github.com wrote:
If you share the text file I can take a look.
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I don't see it, can you share a link to the file?
Can you see the excel file?
On Dec 2, 2016, at 17:43, Luca Pinello notifications@github.com wrote:
I don't see it, can you share a link to the file?
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No, also you need a tsv file to run CRISPRessoPooled per documentation. Excel format is not supported and will not work. Send me the text file you are trying to use not the excel file.
Like this?
On Dec 5, 2016, at 12:23, Luca Pinello notifications@github.com wrote:
No, also you need a tsv file to run CRISPRessoPooled per documentation. Excel format is not supported and will not work. Send me the text file you are trying to use not the excel file.
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CD33 CCCTTCCTCTTTTCTGCTCACACAGGAAGCCCTGGAAGCTGCTTCCTCAGACATGCCGCTGCTGCTACTGCTGCCCCTGCTGTGGGCAGGTGAGTGGCTGTGGGGAGAGGGGTTGTCGGGCTGGGCCGAGCTGACCCTCGTTTCCCCACAGGGGCCCTGGCTATGGATCCAAATTTCTGGCTGCAAGTGCAGGAGTCAGTGACGGTACAGGAGGGTTTGTGCGTCCTCGTGCCCTGCACTTTCTTCCATCCCATACCCTACTACGACAAGAACTCCCCAGTTCATGGTTACTGGTTCCGGGAAGGAGCCATTATATCCAGGGACTCTCCAGTGGCCACAAACAAGCTAGATCAAGAAGTACAGGAGGAGACTCAGGGCAGATTCCGCCTCCTTGGGGATCCCAGTAGGAACAACTGCTCCCTGAGCAT NA NA NA
SIGLEC22P ACATGCCACTGCTGCTACTGCTGCCCCTGCTGTGGGCAGGTGAATGGCTGCGGGGAGAGGGGTTGTCGGGCTGGGCCGAGCTGACCCTCGTTTCCCCACAGGGGCCCTGGCTATGGATCCAAAAATCCGGCTGCAAGTGCAGGAGTCAGTGACGGTACAGGAGGGTTTGTGCGTCCTCGTGCCCTGCACTTTCTCCCATCCCATACCCTACTACAACAGGAATTTCTCAGTTCATGGTTACTGGTTCCGGGAAGGAGCCATTGTATCCAGGGACTCTCCAGTGGCCACAAACAAGCTAGATCAAGAAGTACAGGAGGAGACTCAGGGCTGATTCCGCCTCCTTGGGGATCCCAGTAAGAACAACTGCTCCCTGAGC NA NA NA SIGLEC5 GGAGCATTTCCTTCCCTGACAGCCGGACCTGGGACTGGGCTGGGGCCCTGGCGGATGGAGACATGCTGCCCCTGCTGCTGCTGCCCCTGCTGTGGGGGGGTGAGTGAGCTGAGGGAGGAGGGACAGGCACAGGGGTGAGAAGGGGGGCTGGAGCTGCAGCTGAGCTTCTGTGTCCCCCCAGGGTCCCTGCAGGAGAAGCCAGTGTACGAGCTGCAAGTGCAGAAGTCGGTGACGGTGCAGGAGGGCCTGTGCGTCCTTGTGCCCTGCTCCTTCTCTTACCCCTGGAGATCCTGGTATTCCTCTCCCCCACTCTACGTCTACTGGTTCCGGGACGGGGAGATCCCATACTACGCTGAGGTTGTGGCCACAAACAACCCAGACAGAAGAGTGAAGCCAGAGACCCAG NA NA NA
SIGLEC6 CTCCGCCTCAGAGATGCTACCGCTGCTGCTGCCCCTGCTGTGGGCAGGTGAGTGGGCCAGGGGAGAGGTGCCGTGGGGCTGGGCCGAGCTGACCCTCATGTCTCCATAGGGGCCCTGGCTCAGGAGCGGAGATTCCAGCTGGAGGGGCCAGAGTCACTGACGGTGCAGGAGGGTCTGTGCGTCCTCGTACCCTGCAGATTGCCCACTACCCTTCCAGCCTCGTACTATGGTTATGGCTACTGGTTCCTGGAAGGGGCTGATGTTCCAGTGGCCACAAACGACCCAGACGAAGAAGTGCAGGAGGAGACCCGGGGCCGATTCCACCTCCTCTGGGATCCCAGAAGGAAGAACTGCTCC NA NA NA
SIGLEC12 CTCCTGTGAGGTCACCGTTCAGAACCCACCCCCAACTTCCTGTGCGGCTTCCTCGAAATCTTGAGTTCTCAGTTCCTGAGAGAAGAACCCTGAGGAACAGACTTACCTCAGCAACCCTGGCACCTCCAACCCGACACATGCTACTGCTGCTGCTACTGCTGCCACCCCTGCTCTGTGGGAGAGTGGGGGCTAAGGAACAGAAGGATTACCTGCTGACAATGCAGAAGTCCGTGACGGTGCAGGAGGGCCTGTGTGTCTCTGTGCTTTGCTCCTTCTCCTACCCCCAAAATGGCTGGACTGCCTCCGATCCAGTTCATGGCTACTGGTTCCGGGCAGGGGACCATGTAAGCCGGAACATTCCAGTGGCCACAAACAACCCAGCTCGAGCAGTGCAGGAGGAGACT NA NA NA
SIGLEC17P GCCCTCTTCTCTGCTCACACAGGAAGCCCAGGAAGCCTCTGCCTCAGAGATGCTGCCGCTGCTGCTGCCGCTGCCCCTGCTGTGGGCAGGTGAGTGGCCCTGGGGAGAGGGGCCGTGGGGATGAGCCCATCTGACCCTCATGTCTCCACAGGGCCCTCGCTCAGGATGCAAGATTCCGGCTGGAGATGCCAGAGTCCGTGACGGTGCAGGAGGGTCTGTGCATCTTTGTGCACTGTTCGGTCTTCTACCTCGAGTATGGCTGGAAAGATTCTACCCCTGCTTATGGCCACTGGTTCCGGGAAGGGGTCAGTGTAGACCAGGAGACTCCAGTGGCCACAAACAACTCAACTCAAAAAGTGCAGAAGGAGACCCAGGGCCGATTCCACCTCCTCGGTGATCCCTCAAGGAACAACTGCTCCC NA NA NA
ARHGEF10L CGCCCCATTAGCTTCATGGTGGTTTTCATCACCCCCAACCCCCTGAGCAAGATTTCCTGGGTCAACAGGTTACATTTGGCCAAAATCGGACTCCGTGAGTATAGCCCCAGGGAGAGTGCCTCAGGGGGCAGGGGTGTGGAACGGGGAGGTTTGGGGTGAGTGAGGGTACAGGATGGGTGTTCGGGGTCAGCGGGGGGCAGGGAGGCTTCTGAGTGGGAGGGAGGTGGTGCGGTGGAGAAACAGCTGGGGCCAGAGCCGGAGGCTCTGCTTTGTGACCCTGGACAAGTCACTTCCCCTCTCTGAGCCTCAGCTTCCCCATCCAGGAATTGATGGAACTTGACAGAAAGTAAATTAGTGGTTGCCAGGGACC NA NA NA GHDC GTGCCAGGAGTAGGCAGAAGTGGGCTGTGACAGGTGCATGTGGGCATGGACCCCACAGCCTGGGGCTCAGGAGGGACAAGGAGCTGGGACAGGGTGGATCCCGAGAGAAAGCAAAGCCACTCGGGAGTGGGCAAGAGATATCGTTACGCAGCTGCTGTGTGCCCGACCGACCCTGTCCGAAACCCTTCCTGTACCTTCCCTTCCTCTTCCTTCTTTTCTCCCTTTTTTTTCTTTTCTTTTTTTTTTTGACAGGGTCTCACTCTGTTGCCCAAGCTGGGGTGCAGTGGCACGATCTTGGCTCACTGTAGCCTTGACATCCTGGGCTCAAGGGATCCTCCCATCTCAGCCTCCCAAGTAG NA NA NA VMO1 CTCTCTCACTGCCCCTCCTACCCTAGCTCTGGGAGCCTCTGCCTCCGGGAGCAGGAAATAGGGTTCCGGGAGTGGATTAGTTTTGGGTTTCACTTAATCTGGGAGGCCCTTCCGCAATCGGAGCCCTCACAGAGGCCAAACTGATATAAATCTGCTTAGGAGGCCTGATTCACAGACGCTACAGGATGGAGCGGGGCGCAGGAGCCAAGCTGCTGCCGCTGCTGCTGCTTCTGCGGGCGACTGGTTTCACATGTGCACAGACAGATGGCCGGAACGGCTACACGGCGGTCATCGAAGTGACCAGCGGGGGTCCCTGGGGCGACTGGGCCTGGCCTGAGATGTGTCCCGATGGATTCTTC NA NA NA Chr18 TCTGACAGGCAGGGAAGACTGAGGCAGTGTCTACTGGGCCCTGGTATGAATGGGTCTCTGTGAGGTTAGAAATGGAAGCACGAGTGCAATAGCATTTGTTGCGACTGGAGGGTCAGGCCAAGTCAGAGAGGGTACAGGAAAGTTCGGACTCAAAACCTGGGAAGACACAATGAGGCAAAGATCAAAGATGGGAATTGAAGTTAACTCTGTGAAGCCAAGTGCTCCGAAGGAAGATACCAATATAAGCAGAGTAGGATCAAGACTTAATTGAGTCTTTTGTCCCTGCTTAACGACTCAAGTTAACTTAGCCTCCTCCACCCCTTTTTTTTGGAATGGTATTTCCTTTAAGGACTTTCTTTAAGAAATTCCCGGTGGGG NA NA NA Chr1 GGGGGTAAATAAGACAATCAAGGCAAAATTTTTATACGTGATCTACAAAAATGTCCAAAGAAAGCTCTAAAGGATCACATAGGAGGGAAAGTTTGAGGAAATCTCATAAGGAGGTTACTTTGGTTTGAAATTTGAAAAATCATTAAAGGAAGAGTACAAAAAAGGCTTTGATGCAAGAGAATATGGTTTATAAAATGCCATGTAGCCTAGGATGTCTGGAGTTAGTGTCTGTACAGGAAGGAGAGGTAAGATGGAAAGATTGGTGGAGGCCAGACAGAGGCCCTTATATGCCAGGCTAAAGACTGAATACAATTTTATAGGCAATGAAGAATCATAAAGCACTTTTGAGCAAGAGAGTGGCATGATCA NA NA NA Chr2 TCAGCAAATCTCCTCTTGCTCAACTGAAGATCTTAGGAGGCAAAACAAAATCGATTCATATGGATGCAAGCATTTTCCCACCAGCTGCCACCAGCTCAGGCACAGATGGCTTCTGACACCCATTTGGCTGACAAGGAAAGCCTTAGGGCTGCATTCAAAATGGCTCCAACAGGAGTTGTTAGGAGGTAGAGGAAGTGAAGGGTCTCCAGGCTTAGGAGAGGGAGGCACTGACGGGACAGGATGGGAAGCTGTGGCCCAGCTCCCGAGTGGTCTTTGCACCTGTGACATCGGTTGTGCTTCCTCTGCAGGGGTGGAGTCCAGACTGCGAGGCTGGGCCATCTGCAGGGACCAGCACAGGACAATCAGGTGTCAGGACGTG NA NA NA
Yes something like this, but I need the actual file to see if you use a particular encoding that may be problematic. Please send a link to a file don't copy and paste. You can for example upload it on dropbox, google drive etc and share the link otherwise I cannot help you.
Google drive shared
On Dec 5, 2016, at 12:41, Luca Pinello notifications@github.com wrote:
Yes something like this, but I need the actual file to see if you use a particular encoding that may be problematic. Please send a link to a file don't copy and paste. You can for example upload it on dropbox, google drive etc and share the link otherwise I cannot help you.
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I don't see the link
https://drive.google.com/drive/u/0/folders/0B5PqUF6wEK5YOUtWQVNrVmY3TlU
On Dec 5, 2016, at 12:52, Luca Pinello notifications@github.com wrote:
I don't see the link
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Were you able to get the file?
On Dec 5, 2016, at 12:52 PM, Luca Pinello notifications@github.com wrote:
I don't see the link
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Yes I will take a look soon
I got this error while the amplicon sequence looks fine in the excel.
ERROR: The amplicon sequence ??ރh?????m? contains wrong characters: ? ? ? ? ? ! ? " $ ) ? ? . 0 4 H 9 D ? I ? K ? ? S U O ? ? ] ? ? ? ? ? ? ? ? ? ? ?
Thanks,
Steve