I am trying to install CRISPResso on my computer, but am having trouble using FLASH. Here's the output I get when I try to run the example files:
CRISPResso -r1 reads1.fastq -r2 reads2.fastq -a GCTTACACTTGCTTCTGACACAACTGTGTTCACGAGCAACCTCAAACAGACACCATGGTGCATCTGACTCCTGAGGAGAAGAATGCCGTCACCACCCTGTGGGGCAAGGTGAACGTGGATGAAGTTGGTGGTGAGGCCCTGGGCAGGTTGGTATCAAGGTTACAAGA -e GCTTACACTTGCTTCTGACACAACTGTGTTCACGAGCAACCTCAAACAGACACCATGGTGCATCTGACTCCTGTGGAAAAAAACGCCGTCACGACGTTATGGGGCAAGGTGAACGTGGATGAAGTTGGTGGTGAGGCCCTGGGCAGGTTGGTATCAAGGTTACAAGA
-Analysis of CRISPR/Cas9 outcomes from deep sequencing data-
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[Luca Pinello 2015, send bugs, suggestions or *green coffee* to lucapinello AT gmail DOT com]
Version 1.0.13
WARNING @ Mon, 12 Nov 2018 14:47:51:
Folder CRISPResso_on_reads1_reads2 already exists.
INFO @ Mon, 12 Nov 2018 14:47:51:
Estimating average read length...
INFO @ Mon, 12 Nov 2018 14:47:53:
Merging paired sequences with Flash...
CRITICAL @ Mon, 12 Nov 2018 14:47:53:
Merging error, please check your input.
ERROR: Flash failed to run, please check the log file.
The running log says:
[Execution log]:
Estimating average read length...
Merging paired sequences with Flash...
/bin/sh: /Users/madeleinesitton/CRISPResso_dependencies/bin/flash: cannot execute binary file
Merging error, please check your input.
ERROR: Flash failed to run, please check the log file.
Thank you for the help!!
Hi,
I am trying to install CRISPResso on my computer, but am having trouble using FLASH. Here's the output I get when I try to run the example files:
CRISPResso -r1 reads1.fastq -r2 reads2.fastq -a GCTTACACTTGCTTCTGACACAACTGTGTTCACGAGCAACCTCAAACAGACACCATGGTGCATCTGACTCCTGAGGAGAAGAATGCCGTCACCACCCTGTGGGGCAAGGTGAACGTGGATGAAGTTGGTGGTGAGGCCCTGGGCAGGTTGGTATCAAGGTTACAAGA -e GCTTACACTTGCTTCTGACACAACTGTGTTCACGAGCAACCTCAAACAGACACCATGGTGCATCTGACTCCTGTGGAAAAAAACGCCGTCACGACGTTATGGGGCAAGGTGAACGTGGATGAAGTTGGTGGTGAGGCCCTGGGCAGGTTGGTATCAAGGTTACAAGA