Longer Allele Sequences

[amarcog]

Hello Luca,

Is there any way to have longer allele sequences in the file Alleles_frequency_table_around_cut_sitefor*.txt?

It would be useful for the analysis of HDR events that are located far away from the gRNA cutting site.

Thank you for your attention,

Andrés

[lucapinello]

Yes there is a flag for the command line you can set to have larger alleles. BTW we have a new version of crispresso i.e. Crispresso2. This version is now depracated and not supported anymore.

On Fri, 17 May 2019, 6:59 am amarcog, notifications@github.com wrote:

Hello Luca,

Is there any way to have longer allele sequences in the file Alleles_frequency_table_around_cut_sitefor*.txt?

It would be useful for the analysis of HDR events that are located far away from the gRNA cutting site.

Thank you for your attention,

Andrés

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[amarcog]

Thank you for your answer.

I don't find this flag, is it only available in CRISPResso2? Maybe is --window_around_sgrna? I'm using this version because we want to be consistant with a big amount of old data that was already analyzed with CRISPResso1. But for sure in next experiments I will use the new version.

Than you again for your attention

Andrés

[lucapinello]

Andres,

This is the option:

--offset_around_cut_to_plot 'Offset to use to summarize alleles around the cut site in the alleles table plot.' default=20

Best,

Luca

[amarcog]

Thank you!

Andrés

De: Luca Pinello notifications@github.com Responder a: lucapinello/CRISPResso reply@reply.github.com Fecha: viernes, 17 de mayo de 2019, 15:47 Para: lucapinello/CRISPResso CRISPResso@noreply.github.com CC: amarcog andresmg1993@gmail.com, Author author@noreply.github.com Asunto: Re: [lucapinello/CRISPResso] Longer Allele Sequences (#53)

Andres,

This is the option:

--offset_around_cut_to_plot 'Offset to use to summarize alleles around the cut site in the alleles table plot.' default=20

Best,

Luca

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