Encountered an unknown error during calling.

[gamzemdn]

Hi! I am using the same files as I use with Mutect2 and Varscan. I had no trouble with them but for comparative analysis I ran octopus with same normal-tumor pair using Random Forest option and got the error in the title. I tried different command lines and solutions in the issues but still no luck. Can you help me resolve this? My NORMAL file has SRA code of SRR3182484 and my tumor file has SRR3182485

P.S. I tried without Random forest option and got the same error Thank you

Version octopus 0.5.2-beta

MY command line is octopus -R '/Homo_sapiens/UCSC/hg38/Sequence/WholeGenomeFasta/genome.fa' \ -I normal.bam tumor1recalibratedoutput.bam -N normal \ --somatics-only \ --somatic-forest '/home/gamze/Downloads/octopus_forests_somatic.v0.7.2.forest' \ -o octopus_somatic.vcf.gz \ --debug

I am providing screenshots of cmd as well and log file since it is big.

[dancooke]

Hi, thanks for the bug report. Just to confirm you're using v0.5.2b? This is really old. Can you try a newer version?

[gamzemdn]

Hi I upgraded to v0.6.3b and ran the same command line. This time I got the error below in the log file

Thanks

[gamzemdn]

providing the cmd file as well

[dancooke]

Sorry for the delay in replying to this - v0.6.3b is still old. Please can you try the latest version (v0.7.4). This Bioconda package has just been updated to this version if that's how your installing Octopus.

[gamzemdn]

Hey Dan!

Sorry for the late feedback. Updating resolved the issue, thank you for the guidance, I think we can close the issue now :)

Best wishes,