Open chenzhaozhu opened 1 year ago
Hello, I meet same issue, are you figure out?
Can you please post your command?
On Wed, Jun 14, 2023, 03:42 Wu Ziwei @.***> wrote:
Hello, I meet same issue, are you figure out?
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Yeah, I fixed it. The two files you compare cannot have the same name, and when using nucmer, the reference sequence is followed by the query sequence, which cannot be reversed when merging
Hello, I meet same issue, are you figure out?
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Yeah, I fixed it. The two files you compare cannot have the same name, and when using nucmer, the reference sequence is followed by the query sequence, which cannot be reversed when merging
Can you please post your command?
On Wed, Jun 14, 2023, 03:42 Wu Ziwei @.***> wrote:
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Can you please post your command? … On Wed, Jun 14, 2023, 03:42 Wu Ziwei @.> wrote: Hello, I meet same issue, are you figure out? — Reply to this email directly, view it on GitHub <[https://urldefense.com/v3/__https://github.com/mahulchak/quickmerge/issues/72issuecomment-1590746790;Iw!!CzAuKJ42GuquVTTmVmPViYEvSg!Psz-wygMl3AGFdhw0Lv6ZlFnJf7ISs9fblQoPsvUEpkL93DrA3XHMYYW-JmIUJr01vrqGNSWaSv0P6xVLBD_Z-bv$](https://urldefense.com/v3/__https://github.com/mahulchak/quickmerge/issues/72*issuecomment-1590746790__;Iw!!CzAuKJ42GuquVTTmVmPViYEvSg!Psz-wygMl3AGFdhw0Lv6ZlFnJf7ISs9fblQoPsvUEpkL93DrA3XHMYYW-JmIUJr01vrqGNSWaSv0P6xVLBD_Z-bv%24)>, or unsubscribe <[https://urldefense.com/v3/https://github.com/notifications/unsubscribe-auth/ABZQH2FBRNRMGOY2F2S3U6LXLF2OHANCNFSM6AAAAAAWWHGYM4__;!!CzAuKJ42GuquVTTmVmPViYEvSg!Psz-wygMl3AGFdhw0Lv6ZlFnJf7ISs9fblQoPsvUEpkL93DrA3XHMYYW-JmIUJr01vrqGNSWaSv0P6xVLGmAuFBf$](https://urldefense.com/v3/__https://github.com/notifications/unsubscribe-auth/ABZQH2FBRNRMGOY2F2S3U6LXLF2OHANCNFSM6AAAAAAWWHGYM4__;!!CzAuKJ42GuquVTTmVmPViYEvSg!Psz-wygMl3AGFdhw0Lv6ZlFnJf7ISs9fblQoPsvUEpkL93DrA3XHMYYW-JmIUJr01vrqGNSWaSv0P6xVLGmAuFBf%24)> . You are receiving this because you are subscribed to this thread.Message ID: *@.>
Sure! Here is the command I'd like to share:
source ~/anaconda3/bin/activate ~/anaconda3/envs/genome PPN=2 genome_file1=/public1/home/genome/nextdenovo/03.ctg_graph/nd.asm.fasta genome_file2=/public1/home/polish_genome/genome.nextpolish.fasta output_file=next+flye nucmer -l 100 -prefix ${output_file} ${genome_file2} ${genome_file1} delta-filter -r -q -l 10000 ${output_file}.delta > ${output_file}.rq.delta quickmerge -d ${output_file}.rq.delta -q ${genome_file1} -r ${genome_file2} -hco 5.0 -c 7.0 -l n -ml m -p ${output_file} Thanks for your help!
1 -d 2 2020merge/out.rq.delta 3 -q 4 2020.fa 5 -r 6 pilon_polished.fasta 7 -hco 8 5.0 9 -c 10 1.5 11 -l 12 0 13 -ml 14 5000 15 -p 16 2020merge/2020.mfa ptg000009l_pilon ptg000009l_pilon 1 JAAIVE010000736.1 1 ptg000536l_pilon -1 ptg000091l_pilon JAAIVE010000152.1 1 ptg000091l_pilon -1 ptg000199l_pilon ptg000199l_pilon 1 JAAIVE010000169.1 -1 ptg000755l_pilon -1 ptg000285l_pilon ptg000285l_pilon 1 JAAIVE010000015.1 -1 ptg001264l_pilon -1 ptg000294l_pilon JAAIVE010000686.1 1 ptg000294l_pilon 1 ptg000341l_pilon ptg000341l_pilon 1 JAAIVE010000429.1 -1 ptg000036l_pilon 1 ptg000547l_pilon ptg000547l_pilon 1 JAAIVE010000200.1 -1 ptg000565l_pilon ptg000565l_pilon 1 JAAIVE010000017.1 -1 ptg000682l_pilon 1 ptg001402l_pilon ptg001402l_pilon 1 JAAIVE010000003.1 1 ptg000326l_pilon 1 terminate called after throwing an instance of 'std::out_of_range' what(): basic_string::substr: __pos (which is 20474) > this->size() (which is 0)