Hi! I have been using this tool to do sequence-graph alignments between a reference sequence and fragmented assemblies (I'm trying to retrieve a full novel gene through traversing graph paths), and it's been super helpful for sequences that have high nucleotide identity to my references (80-90+%).
I also have some sequences that are in the 50% amino acid identity range, which become too diverged to use nucleotide alignment for sequence-graph alignment. I was wondering if it would be possible to implement a feature that accepts amino acid sequences as input reference? (I am not too familiar with tool development so maybe this is an impossible ask 😅)
Hi! I have been using this tool to do sequence-graph alignments between a reference sequence and fragmented assemblies (I'm trying to retrieve a full novel gene through traversing graph paths), and it's been super helpful for sequences that have high nucleotide identity to my references (80-90+%).
I also have some sequences that are in the 50% amino acid identity range, which become too diverged to use nucleotide alignment for sequence-graph alignment. I was wondering if it would be possible to implement a feature that accepts amino acid sequences as input reference? (I am not too familiar with tool development so maybe this is an impossible ask 😅)