mal-boisestate / Immunostained_Image_Analysis

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Count cell numbers on 10x or 20x DIC images #38

Open ninanikitina opened 1 year ago

ninanikitina commented 1 year ago

As a researcher, I want to use a program to count cell numbers on 10x or 20x DIC images so that I can analyze cell proliferation without using DAPI dye that negatively affects the cell cycle.

ninanikitina commented 1 year ago

Image

ninanikitina commented 1 year ago

Dr.Uzer suggested using DAPI channel and our 20x model to create a training set. So we can test two ways:

  1. DAPI -> OLD_20x_model -> MASKS | DIC + MASKS -> train new model
  2. DIC + manual MASKS with augmentation -> train new model
Ben-Ben-M commented 1 year ago

Will try current model on 10x img today. May attempt to train a new 10x-specific model, for which we'll need a training sample.