Open manulera opened 1 year ago
Example from https://doi.org/10.1242/jcs.001115
The cdc8-AS allele was created by PCR using primers oDM109 (5′-CATATGGCTAGCATGG-ATAAGCTTAGAGAGAAAAT-3′) and oDM112, cloned into pTOPO2.1 and sequenced (creating pTOPOcdc8-AS). The cdc8+ and cdc8-AS alleles were subsequently cloned into the pJC20 bacterial T7 expression vector.
Especially for allele description in papers where the plasmid is given by name but sequence can't be found, if the plasmid has only been used to clone a fragment, and then clone it out.