Parsnp was designed to align the core genome of hundreds to thousands of bacterial genomes within a few minutes to few hours. Input can be both draft assemblies and finished genomes, and output includes variant (SNP) calls, core genome phylogeny and multi-alignments. Parsnp leverages contextual information provided by multi-alignments surrounding SNP sites for filtration/cleaning, in addition to existing tools for recombination detection/filtration and phylogenetic reconstruction.
The --extend-lcbs parameter now performs a gapped alignment using either muscle (>50nt) or abpoa (<=50nt) on inter-LCB regions. Alignments are trimmed back based on an ANI parameter that can also be provided by the user (i.e., cuts alignment off when it falls below 95% ANI). This parameter still only works w/ single contig genomes.
Also added better SeqIO validation. Throws out any genomes that can't be parsed.
The
--extend-lcbsparameter now performs a gapped alignment using either muscle (>50nt) or abpoa (<=50nt) on inter-LCB regions. Alignments are trimmed back based on an ANI parameter that can also be provided by the user (i.e., cuts alignment off when it falls below 95% ANI). This parameter still only works w/ single contig genomes.Also added better
SeqIOvalidation. Throws out any genomes that can't be parsed.