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how to use Treemix_bootstrap.sh if more than one population is choosen as outgroup #5

Closed Carol-Symbiomics closed 2 years ago

Carol-Symbiomics commented 3 years ago

Dear BITE developers,

I would like to know if your bash script supports more than 1 population ID as an outgroup?

While running Treemix directly I have no trouble setting the root to a group of populations as follows treemix -i spis.18reefs.pop.TM.gz -root FAR_R1,FAR_R2,FAR_R3,FAR_R4 -o spis.18reef.allmarker_rootedFAR > rootedFAR_log

However, when I try to accomplish the same tree rooting strategy using Treemix_bootstrap.sh using the following command: Treemix_bootstrap.sh spis.18reefs.pop.TM.gz 11 40 500 FAR_R1,FAR_R2,FAR_R3,FAR_R4 100 /home/buitracn/RADseq/tools/phylip-3.697/exe/consense spis.18reefs.pop.allmarkers.FARrooted.m11.100boots.TM

I get the following error: ERROR in placing root: no clade FAR_R1,FAR_R2,FAR_R3,FAR_R4

Please advise!