I know if I want to train a model, I should first use tombo to resquiggle fast5 files. And then convert them to numpy array.
But if I want to basecall a fast5 file, should I use tombo to resquiggle fast5 files? Because I think I should not use the ground truth reference gnome to act as the tombo reference. But the combo need a reference patameter.
Hello,
I know if I want to train a model, I should first use tombo to resquiggle fast5 files. And then convert them to numpy array.
But if I want to basecall a fast5 file, should I use tombo to resquiggle fast5 files? Because I think I should not use the ground truth reference gnome to act as the tombo reference. But the combo need a reference patameter.