martinfthomsen
commented
1 year ago - resolve issue #18 - Add qPCR support. The identification and use of hybridisation probes are now fully supported throughout RUCS
- resolve issue #13 - Make reported positions relative to reference
- resolve issue #15 - Support for specifying work dir and result dir
- resolve issue #20 - use accessions ids as display names on Virtual PCR result
- improve Virtual PCR - both performance and output
- Improve sorting of primer pairs by upgrading the penalty scoring algorithm
- upgrade Primer3-py module and update parser
- integrate the genome download script and support accession IDs as direct input
- add support for downloading nucleotide sequence from NCBI using accession IDs
- improve reusability of intermediate results for performance boost of reruns
- Add entrypoint tests script and corresponding test data
- move test data from test/ to testdata/
- reorganise output files into inputs, work and results directories
- switch from 3 to 4 spaces indentation
- bugfixes
- update default settings
- update documentation
- update readme