Please provide a feature for generating consensus sequences based on k-mers which maps to a specified percentage of the positive references, and which does not map to a minimum specified percentage of negative references.
This might alleviate the issue when the positive strains are too distantly related to provide enough consensus material, or when some negative references are too closely related. Thus, removing all potential material for identifying PCR primers...
Please provide a feature for generating consensus sequences based on k-mers which maps to a specified percentage of the positive references, and which does not map to a minimum specified percentage of negative references.
This might alleviate the issue when the positive strains are too distantly related to provide enough consensus material, or when some negative references are too closely related. Thus, removing all potential material for identifying PCR primers...