Hi,wenjing.Bother you again.When I use Cellcano preprocess to create a gene matrix from the data, an error is reported, the specific content is as follows.I don't know if it's a problem with the file path or something else. Can you check it out for me?thank you.
R[write to console]: Saving ArchRProject...
R[write to console]: ArchR logging to : ArchRLogs/ArchR-getMatrixFromProject-12b345949cca7-Date-2023-08-25_Time-11-08-57.03071.log
If there is an issue, please report to github with logFile!
R[write to console]: Error in h5checktypeOrOpenLoc(file, readonly = TRUE, fapl = NULL, native = native) :
Error in h5checktypeOrOpenLoc(). Cannot open file. File 'NA' does not exist.
Traceback (most recent call last):
File "/data/houjy/anaconda3/envs/cellcano/bin/Cellcano", line 8, in <module>
sys.exit(main())
File "/data/houjy/anaconda3/envs/cellcano/lib/python3.8/site-packages/Cellcano/main.py", line 107, in main
preprocess.preprocess(args)
File "/data/houjy/anaconda3/envs/cellcano/lib/python3.8/site-packages/Cellcano/preprocess.py", line 156, in preprocess
_run_ArchR(input_files, sample_names, output_dir=output_dir)
File "/data/houjy/anaconda3/envs/cellcano/lib/python3.8/site-packages/Cellcano/preprocess.py", line 69, in _run_ArchR
ArchR_genescore_mat = ArchR_genescore_func(ArchR_proj, useMatrix="GeneScoreMatrix")
File "/data/houjy/anaconda3/envs/cellcano/lib/python3.8/site-packages/rpy2/robjects/functions.py", line 208, in __call__
return (super(SignatureTranslatedFunction, self)
File "/data/houjy/anaconda3/envs/cellcano/lib/python3.8/site-packages/rpy2/robjects/functions.py", line 131, in __call__
res = super(Function, self).__call__(*new_args, **new_kwargs)
File "/data/houjy/anaconda3/envs/cellcano/lib/python3.8/site-packages/rpy2/rinterface_lib/conversion.py", line 45, in _
cdata = function(*args, **kwargs)
File "/data/houjy/anaconda3/envs/cellcano/lib/python3.8/site-packages/rpy2/rinterface.py", line 817, in __call__
raise embedded.RRuntimeError(_rinterface._geterrmessage())
rpy2.rinterface_lib.embedded.RRuntimeError: Error in h5checktypeOrOpenLoc(file, readonly = TRUE, fapl = NULL, native = native) :
Error in h5checktypeOrOpenLoc(). Cannot open file. File 'NA' does not exist.
Hi,wenjing.Bother you again.When I use
Cellcano preprocess
to create a gene matrix from the data, an error is reported, the specific content is as follows.I don't know if it's a problem with the file path or something else. Can you check it out for me?thank you.