matanhofree / crc-immune-hubs

Principal analysis code used in for analysis of single cell RNAseq of primary untreated colon cancer. As described in: "Spatially organized multicellular immune hubs in human colorectal cancer"
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Confused by the NMF clustering and program identification #1

Open Sudolin opened 2 years ago

Sudolin commented 2 years ago

Dear Authors,

Great work and Thanks for sharing your data and code.

After reading the methodology, I find myself confused by the way you used NMF to cluster cells and infer programs. Could you elaborate more on this part? Are the programs used to help cluster cells the same as those to infer programs?

Thanks in advance.