Tm can not be computed

[k1197445]

Hi, I followed the tutorial trying to compute the Tm of primers, but failed. Could you have a look at the reason? Many thanks

constraint.df = check_constraints(primer.df, template.df, settings, active.constraints = "melting_temp_range") Error in read.table(file = file, header = header, sep = sep, quote = quote, : no lines available in input In addition: Warning message: executing %dopar% sequentially: no parallel backend registered Warning messages: 1: In call.melt.single(out.primers[seq.idx[[i]]], out.complements[seq.idx[[i]]], : MELTING could not compute the Tm: File: /tmp/RtmpMIHvTh/melt_seqs_1b7d69d6c4dc346a04f0544b03a6093ea6cf555175893.txt.results.csv Error in read.table(file = file, header = header, sep = sep, quote = quote, : no lines available in input

2: In min(c(Tm.fw$Tm_C_fw[x], Tm.rev$Tm_C_rev[x]), na.rm = TRUE) : no non-missing arguments to min; returning Inf 3: In min(c(Tm.fw$Tm_C_fw[x], Tm.rev$Tm_C_rev[x]), na.rm = TRUE) : no non-missing arguments to min; returning Inf

[k1197445]

To add, I used R3.6.

[matdoering]

Which version of MELTING is installed on your system?

Works for me with version 5.1

You can check the version via:

melting -V

Can you open the file that is located in /tmp/? What are the contents?

[k1197445]

Thanks. Problem solved.