search

maweigert / biobeam

GPU accelerated beam propagation tools for biological light tissue simulations
BSD 3-Clause "New" or "Revised" License
35 stars 9 forks source link

unit of units-parameter in nanometers and not microns? #2

Closed haesleinhuepf closed 6 years ago

haesleinhuepf commented 6 years ago

Hey Martin,

when playing with the method biobeam.focus_field_beam, we had the feeling, that the units parameters expects values in nanometers, but according to the documentation, it's microns. Can you clearify?

Thanks, Robert

maweigert commented 6 years ago

units should be all given in microns, so the following should give you the widefield psf on a grid of pixlsize 0.1 um:

u = focus_field_beam(shape = (128,)*3,units = (.1,)*3, lam = .5, NA = .8)

Is that what you want?

haesleinhuepf commented 6 years ago

Thanks for the example! I found our bug: We entered the wavelength (lam) in nanometers and that's why the PSF looked so strange!

Thanks a lot!!

https://github.com/haesleinhuepf/biobeam_examples/blob/master/Advanced_microscopy_course_1.ipynb