Closed maxibor closed 4 years ago
Alternatively, you could rely on a program that allows to have a third adapter sequence that is introduced by the transposase tagmentation step in the Illumina Nextera XT library prep kit.
I used trim_galore
(https://github.com/FelixKrueger/TrimGalore/blob/master/Docs/Trim_Galore_User_Guide.md), which itself uses cutadapt
.
Here some example code:
trim_galore --paired \
--quality 10 \
--phred33 \
--nextera \
--gzip \
--length 35 \
--output_dir {params.outdir} \
--basename {wildcards.sample} \
--cores {threads} \
{input.pe1} {input.pe2}
AdapterRemoval also has the option to use an file containing a list of adapters --adapter-list
Now implemented with 51cec64
Give the user the possibility to provide an adapter list