maximilianh
commented
4 years ago softmasked. Crispor needs the normal genome.
The softmasking is useful, e.g. in primer design, we can avoid repeats. As a general rule, softmasked genomes should be the default everywhere. There is no loss of information and it's often good to know about repeats.
On Wed, Jul 17, 2019 at 5:12 AM hope notifications@github.com wrote:
Dear, I want to add my research genome to crispor. And I noted the example in Adding a genome https://github.com/maximilianh/crisporWebsite#adding-a-genome section used a repeatMasked genome fasta file. My question is why not an original assembled genome. And original assembled genome, hardmasked and softmasked, which is best?
Any help is much appreciated. Thanks.
Best regards
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tiramisutes
Dear, I want to add my research genome to crispor. And I noted the example in Adding a genome section used a repeatMasked genome fasta file. My question is why not an original assembled genome. And original assembled genome, hardmasked and softmasked, which is best?
Any help is much appreciated. Thanks.
Best regards