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maximilianh / crisporWebsite

All source code of the crispor.org website
http://crispor.org
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Whether the input genome should be repeat masked? #41

Closed tiramisutes closed 4 years ago

tiramisutes commented 4 years ago

Dear, I want to add a genome in "fasta" mode use crisprAddGenome fasta command. Then I see the Adding a genome, but I want to know whether the fasta genome should be repeated masked?

Thanks.

maximilianh commented 4 years ago

No. Please do not repeat mask the genome. It's good to have it soft-masked, so primer3 can avoid repeats. But you want to know about CRISPR off-targets in repeats.

Also, it's easier if you send me a genome so I can index it. I can block it for downloads on the website.

On Mon, Dec 30, 2019 at 4:05 PM hope notifications@github.com wrote:

Dear, I want to add a genome in "fasta" mode use crisprAddGenome fasta command. Then I see the Adding a genome https://github.com/maximilianh/crisporWebsite#adding-a-genome, but I want to know whether the fasta genome should be repeated masked?

Thanks.

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