Closed sunta3iouxos closed 4 months ago
Hi,
the default spikein_bin_size
for calculating scaling factors from spikein genome is 1000 . This should be visible in the workflow config.yaml in the output folder.
In the first commandline you pasted, I've noticed you are passing host bam files to calculate spikein size factors. Then you're citing size factors from a file, in which they appear to be calculated on spikein bam files. Could you revisit that?
If you run snakePipes with --verbose
, full shell commands will be returned in the log file. You can then see the full multiBamSummary
command with all the parameters passed. Would that be helpful?
Best,
Katarzyna
In the first commandline you pasted, I've noticed you are passing host bam files to calculate spikein size factors. Then you're citing size factors from a file, in which they appear to be calculated on spikein bam files. Could you revisit that?
that was a typo from my side, I will come back after testing a few things
I am getting different results using the multiBamSummary command on my local PC using the following command that I expected to be the same on the server side, and I am suspecting that this is probably the issue: my command:
and the output is as follows:
Using a different --binSize to recapitulate the Number of bins found, did not solved the issue: