mbhall88 / head_to_head_pipeline

Snakemake pipelines to run the analysis for the Illumina vs. Nanopore comparison.
GNU General Public License v3.0
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Convert single-fast5 to multi-fast5 #30

Closed mbhall88 closed 4 years ago

mbhall88 commented 4 years ago
mbhall88 commented 4 years ago

birmingham-mgit-run1 and birmingham-mgit-run2 seem to missing nearly all of the original fast5 data. After unarchiving the fast5s there was on about 3000 single fast5 files. However, the fastqs that came with the files indicate there should be far more reads than that. Martin has checked on the Oxford servers and they seem to have deleted the fast5 data on their side.

We either need to use their fastq files, or exclude the samples involved in these runs (11 samples). The only thing is, I don't know what version of guppy the data has been basecalled with. Seeing as we received this data in 2018, I suspect it is much older than the version we are using.

mbhall88 commented 4 years ago

We will just write off the two runs with missing fast5