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mcanouil / NACHO

NAnostring quality Control dasHbOard
https://m.canouil.dev/NACHO/
GNU General Public License v3.0
8 stars 4 forks source link

PlexSet Analysis #30

Closed yanwu2014 closed 2 years ago

yanwu2014 commented 2 years ago

Hey guys,

This looks like an awesome tool! I was wondering if NACHO supported PlexSet analysis where each well/lane is multiplexed with up to 8 samples? If so, it looks like the load_rcc function requires each sample to be a separate file so should we demultiplex each well/lane?

Thanks in advance for your help! Yan

mcanouil commented 2 years ago

Hi,

Unless NanoString made a major update of their RCC file format, multiplex data are the same as regular data. The difference is instead of having "Endogenous", you have "Endogenous1", ..., "Endogenous8" for multiplex, which is parsed and read by NACHO.

For short, it worked before, thus should work now. If not, please fill an issue with a reproducible example (fake RCC file close to yours for example)