Open gnapham opened 4 years ago
Hi gnapham,
Totally; this has been requested a couple of times, I'll try to make some headway on this, though it'll take a bit of time. I'll check back when I have something here. Thanks,
Aaron
Hi, are there still plans for custom PAM sequences or alternative enzymes?
Hi charlesfeigin,
Slow progress on it. Right now the big issue is that we store targets in 48 bits, so we're limited to 24 basepair targets. Version 2 is in the works that will support longer target sequences. If your custom target (+ PAM) is 24 bases or less we can add support for that pretty quickly, but longer targets will require the changes in V2.
-Aaron
I'd just like to add my support for the custom target length (especially for Cpf1 - I'm interested in 27nt = 23 + PAM).
Very nice tool by the way.
I'd also like to add my support for longer/custom target sequences. @aaronmck, is there an ETA on version 2?
Hi @aaronmck, any news about this feature? Thanks 🙏
Hi @aaronmck, I have been following this issue and it would be absolutely beneficial if Flashfry could have custom PAM sites.
Is it possible to add functionality to build the off target database using custom PAM sequences?