Hi - we have been working on designing gRNAs with alternate PAM sites. One Cas enzyme we have been testing is the SpG Cas enzyme. This is an engineered version of the Cas9 enzyme that recognizes an NG PAM site. I was wondering if you can provide an additional option to index and discover guides using this enzyme.
Hi - we have been working on designing gRNAs with alternate PAM sites. One Cas enzyme we have been testing is the SpG Cas enzyme. This is an engineered version of the Cas9 enzyme that recognizes an NG PAM site. I was wondering if you can provide an additional option to index and discover guides using this enzyme.