mckennalab / FlashFry

FlashFry: The rapid CRISPR target site characterization tool
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Customizable PAM Sequence for Cpf1 (e.g., TTTV instead of Default TTTN) #41

Open 2021JohnSheng opened 1 month ago

2021JohnSheng commented 1 month ago

Hi, Thank you for your continuous efforts in maintaining and improving this software. It has been a valuable tool for our research. Currently, the software supports the default Cpf1 PAM sequence TTTN. However, our research requires the flexibility to use a different PAM sequence, specifically TTTV (where V represents A, C, or G). Could you please add a feature that allows users to customize the PAM sequence, including support for degenerate bases like TTTV? Thank you for considering this request.