Closed hcdenbakker closed 7 years ago
Hey Henk
in your first line you seem to have two samples, one with .:. and one with 0:0 - what does this mean? What is .:. in this line, I thought it was coverage?
The entry which genotypes the sample as 1/1 also has a zero for GQ - so there is no confidence in that genotype.
re 1: .:. is a remnant of a dummy sample (needed to introduce this to get a vcf with all variable sites for an entire population), the last column represents a real sample/coverage
re 2: Overlooked the zero GQ... thanks for pointing that out. I let the 1/1 genotype call distract me.
On Thu, Apr 20, 2017 at 6:52 PM, Zamin Iqbal notifications@github.com wrote:
Hey Henk
1.
in your first line you seem to have two samples, one with .:. and one with 0:0 - what does this mean? What is .:. in this line, I thought it was coverage? 2.
The entry which genotypes the sample as 1/1 also has a zero for GQ - so there is no confidence in that genotype.
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I was looking into vcf-genotyping and introduced a zero coverage locus:
CP006053.1 4399342 . A C . PASS BUBBLE=204;K33 K33R:K33A .:. 0:0
only to find it was called as the alternative allele when genotyped:
CP006053.1 4399342 . A C . PASS BUBBLE=204;K33 GT:K33R:K33A:GQ ./.:.:.:. 1/1:0:0:0
I used this command:
./mccortex63 vcfgeno --out out.vcf.gz --kcov 30 --ploidy 2 myvcf.cov.vcf