The otsu thresholding for fluorescence segmentation extended for 3D volume segmentation in preprocessing module. This avoids the deconvolution artifacts in out-of-plane slices. The normalization of slices was performed based on intensity range of center slice and simple thresholding was perform from the threshold value of center slice.
The otsu thresholding for fluorescence segmentation extended for 3D volume segmentation in preprocessing module. This avoids the deconvolution artifacts in out-of-plane slices. The normalization of slices was performed based on intensity range of center slice and simple thresholding was perform from the threshold value of center slice.