Severe artifacts and blur in 2D phase reconstruction

mehta-lab / waveorder

Wave optical models and inverse algorithms for label-agnostic imaging of density & orientation.

BSD 3-Clause "New" or "Revised" License

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Open ziw-liu opened 1 week ago

ziw-liu commented 1 week ago

Reconstructing 2D phase introduces checkerboard artifacts and blur (20x 0.55NA):

/hpc/projects/comp.micro/virtual_staining/datasets/iNeurons/20240416_Exp01_iN_day7_calcein_hoescht/1-reconstruction/phase_2d.yaml

3D phase:

The same problem was previously found in an OPS experiment (5x 0.13NA).

ziw-liu commented 1 week ago

Possibly related to #152.