I am trying to demultiplex an Illumina run using iu-demultiplex, here is my command iu-demultiplex -s SampleSheet-RC.txt --r1 lane1_NoIndex_L001_R1_001-13C.fastq.gz --r2 lane1_NoIndex_L001_R3_001-13C.fastq.gz --index lane1_NoIndex_L001_R2_001-13C.fastq.gz -o output/
But I got following errors:
Output directory .............................: /Users/Jincheng/Desktop/tmp/output
Barcodes .....................................: 13 samples found
Traceback (most recent call last):
File "/Users/Jincheng/miniconda3/envs/py34/bin/iu-demultiplex", line 238, in
d._run()
File "/Users/Jincheng/miniconda3/envs/py34/bin/iu-demultiplex", line 45, in _run
self.build_index()
File "/Users/Jincheng/miniconda3/envs/py34/bin/iu-demultiplex", line 116, in build_index
progress.update('~%.2f%% (num index reads with no barcode: %d (%.2f%% of all reads))' % (self.index.percent_read, missing_barcode, missing_barcode * 100.0 / num_index))
TypeError: a float is required
Dear developer,
I am trying to demultiplex an Illumina run using iu-demultiplex, here is my command
iu-demultiplex -s SampleSheet-RC.txt --r1 lane1_NoIndex_L001_R1_001-13C.fastq.gz --r2 lane1_NoIndex_L001_R3_001-13C.fastq.gz --index lane1_NoIndex_L001_R2_001-13C.fastq.gz -o output/But I got following errors: Output directory .............................: /Users/Jincheng/Desktop/tmp/output Barcodes .....................................: 13 samples found
d._run()
File "/Users/Jincheng/miniconda3/envs/py34/bin/iu-demultiplex", line 45, in _run
self.build_index()
File "/Users/Jincheng/miniconda3/envs/py34/bin/iu-demultiplex", line 116, in build_index
progress.update('~%.2f%% (num index reads with no barcode: %d (%.2f%% of all reads))' % (self.index.percent_read, missing_barcode, missing_barcode * 100.0 / num_index))
TypeError: a float is required
Traceback (most recent call last): File "/Users/Jincheng/miniconda3/envs/py34/bin/iu-demultiplex", line 238, in
Could you help? Thank you!
Jincheng