Open mariabernard opened 4 years ago
Maria, the error message says use --ignore-deflines
if none of this makes any sense. Did you try doing that?
I could not find this option in the program help section.
I tried
iu-demultiplex --ignore-deflines -s sample_barcode.txt --r1 mock-forward-read.fastq.gz --r2 mock-reverse-read.fastq.gz --index mock-index-read.fastq -o output/
and I received this error
iu-demultiplex: error: unrecognized arguments: --ignore-deflines
Dear all,
I am trying to use iu-demultiplex to work with those fastq files : https://github.com/caporaso-lab/mockrobiota/blob/master/data/mock-9/dataset-metadata.tsv
but it's seems to no be FASTQ file generated by CASAVA 1.8, as iu-demultiplex return me this error :
and I don't undertstand " 'raw = True' parameter ", as it's not an option of iu-demultiplex. Could you tell me if it's possible to use your program with those data ?
They looks like this
(its not always NNNNNNN in the index file)
Kind regards
Maria