Normalization how it is implemented now normalizes :
the single end/merged paired-end reads, but takes the unnormed paired end reads into account.
the paired end reads but takes the unnormed single end reads into account.
To me this seems like two biased normalizations.
Megahit shout be able to handle very different abundant metagenomes. Doesn't help for the assembly?Spades has a internal normalization function, so we could use this one instead of bbnorm.
Normalization how it is implemented now normalizes :
To me this seems like two biased normalizations.
Megahit shout be able to handle very different abundant metagenomes. Doesn't help for the assembly?Spades has a internal normalization function, so we could use this one instead of bbnorm.