metagenome-atlas / atlas

ATLAS - Three commands to start analyzing your metagenome data
https://metagenome-atlas.github.io/
BSD 3-Clause "New" or "Revised" License
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Progressing despite individual binner failure #651

Open mladen5000 opened 1 year ago

mladen5000 commented 1 year ago

Here is the relevant log output:

My issue pertains to binning -> genome creation. I often have smaller datasets so I am interested in performing ensemble binning [metabat, maxbin, vamb, and semibin], however, many times there are 1,2, even 8 samples that don't recover bins. It seems if using das tool this should be ok. Instead I typically resort to attempting all 4 methods but almost always am required to stick to metabat as the final binner.

Perhaps touching a temp file (results header, just empty for example) when binning is complete is possible?

Atlas version 2.16.1 Additional context Add any other context about the problem here.

SilasK commented 1 year ago

Can you tell me which binner you used? maxbin?

Would you be ok to simply not bin this sample?

You could still use it to be quantified..

mladen5000 commented 1 year ago

I used initially wanted to use all 4 available and aggregate with DASTools. Maxbin however is the one where this most frequently occurs. I think the most convienent option would be to have the workflow continue without having to manually curate the config and samples (Currently I end up removing maxbin, then removing either vamb or Semibin if they cause issues, then switching the final binner to metabat, and finally dropping samples from samples.tsv).

If there's a way to not bin but still quantify that would be great

github-actions[bot] commented 11 months ago

There was no activity since some time. I hope your issue is solved in the mean time. This issue will automatically close soon if no further activity occurs.

Thank you for your contributions.