metagentools / MetaCoAG

🚦🧬 Binning Metagenomic Contigs via Composition, Coverage and Assembly Graphs
https://metacoag.readthedocs.io/en/stable/
GNU General Public License v3.0
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coverage across multiple samples for binning #44

Closed franciscozorrilla closed 2 weeks ago

franciscozorrilla commented 3 months ago

Hello, thanks for developing this tool.

In the metacoag paper you write:

MetaCoAG can be easily extended to work with other metagenomics assemblers. In the future, we plan to extend MetaCoAG to support overlapped binning [24] (i.e., contigs may belong to multiple species) and multi-sampled binning [27] (i.e., integration across multiple samples instead of co-assembly).

I am wondering if the current implementation of metacoag supports input coverage across multiple samples for binning?

Best, Francisco

Vini2 commented 2 months ago

Hi @franciscozorrilla,

Thanks for your interest in MetaCoAG.

Yes, MetaCoAG supports input coverage across multiple samples for binning. You can generate coverage files for each sample using a tool such as CoverM and combine those into one abundance file using the combine_cov.py script.

Please let me know if you have any further questions.

Thanks, Vijini

Vini2 commented 2 weeks ago

Closing this issue as coverage across multiple samples is already supported. Please re-open if there are further issues on this topic.

franciscozorrilla commented 2 weeks ago

Thanks for the info @Vini2 ! Will re-open if I have any issues with this.