new children of abnormal CD4-positive T cell morphology

[obophenotype-user]

Under term "abnormal CD4-positive T cell morphology" could we add

1. decreased CD25-positive T cell number Definition: a reduction in the number of the subset of T lymphocytes that carry the CD4 and CD25 marker, which regulate overall immune responses as well as the responses of other T cell subsets through direct cell-cell contact and cytokine release
2. increased CD25-positive T cell number Definition: a greater number of the subset of T lymphocytes that carry the CD4 and CD25 marker, which regulate overall immune responses as well as the responses of other T cell subsets through direct cell-cell contact and cytokine release ?

Reported by: natashakarp

Original Ticket: obo/mammalian-phenotype-requests/603

[obophenotype-user]

I will need to consult the developers of the cell type ontology to see if the term CD25-positive T cell number fits with the definition of "CD4-positive, CD25-positive, alpha-beta regulatory T cell morphology". I think that all CD4-positive, CD25-positive T cells are alpha-beta T cells, but I will need to confirm this.

Original comment by: cindyJax

[obophenotype-user]

• assigned_to: nobody --> cynthiasmith
• status: open --> pending

Original comment by: cindyJax

[obophenotype-user]

I am reluctant to absolutely assert that all CD4-positive, CD25-positive T cells are alpha-beta T cells (since it's not inconceivable that this combination of markers could occur on some rare gamma-delta T cells), but certainly the regulatory T cells that are studied to a great degree these days are. If a paper was studying CD4+, CD25+ T cells and they were something other than alpha-beta T cells, it would surely be mentioned, since it would normally be assumed they are alpha-beta T cells.

You should rename and redefine the term according to the Cell Ontology in any case, to eliminate any ambiguity.

Thanks,

Alex

Original comment by: addiehl

[obophenotype-user]

The cells that I find with CD25-positive in the name in the CL is "CD4-positive, CD25-positive, alpha-beta regulatory T cell morphology" CL:0000792 and children of this term.

However, I also see "activated CD4-positive, alpha-beta T cell" CL:0000896 defined as "A recently activated CD4-positive, alpha-beta T cell with the phenotype CD69-positive, CD62L-negative, CD127-negative, and CD25-positive." [GO_REF:0000031, GOC:add, GOC:pam]

The definition listed by Natasha suggests that regulatory T cells are correct, but I suspect that I will need more information from her to make the correct determination.

Original comment by: cindyJax

[obophenotype-user]

Yes, CD25 also appears transiently as an activation marker on T cells that are not necessarily regulatory T cells. The presence of the Foxp3 transcription factor is a more definitive marker of regulatory T cells, but many researchers rely on the CD4/CD25 combination because these markers can be seen on live cells to allow for cell sorting, and are generally easier to assay by flow cytometry.

CD25 expression on regulatory T cells is considered to be more stable.

-- Alex

Original comment by: addiehl

[obophenotype-user]

Since the proposed definitions match with the CL:0000792 term for "CD4-positive, CD25-positive, alpha-beta regulatory T cell" we have added the following new terms:

increased CD4-positive, CD25-positive, alpha-beta regulatory T cell number MP:0010168

decreased CD4-positive, CD25-positive, alpha-beta regulatory T cell number MP:0010169

Please let us know if this is not correct and we will amend as necessary.

Original comment by: cindyJax

[obophenotype-user]

• status: pending --> closed-accepted

Original comment by: cindyJax