Thank you for your interest in the software.
Can you elaborate on your question? Are you referring to gene-derived siRNA, or TE-derived siRNA?
In theory, the software identifies small RNA that align to either gene or TE, and consider them small RNA derived from a particular feature (gene/TE). You then need to perform other downstream analyses to truly define them as siRNA (e.g. looking at their distribution along the gene/TE, size profile etc). Thus, while TEsmall will provide you with counts of small RNA derived from particular features, the more strict siRNA definition needs further analyses to confirm.
Hi,
Thank you for your interest in the software. Can you elaborate on your question? Are you referring to gene-derived siRNA, or TE-derived siRNA?
In theory, the software identifies small RNA that align to either gene or TE, and consider them small RNA derived from a particular feature (gene/TE). You then need to perform other downstream analyses to truly define them as siRNA (e.g. looking at their distribution along the gene/TE, size profile etc). Thus, while TEsmall will provide you with counts of small RNA derived from particular features, the more strict siRNA definition needs further analyses to confirm.
Thanks.