mhammell-laboratory / TEtranscripts

A package for including transposable elements in differential enrichment analysis of sequencing datasets.
http://hammelllab.labsites.cshl.edu/software/#TEtranscripts
GNU General Public License v3.0
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mapping gtf version different with TEtranscripts version #166

Closed songlyzz closed 5 months ago

songlyzz commented 6 months ago

Hi olivertam, I I'd like to ask for advice that I use gencode v25 gtf to map, the version is different with GRCh38_Ensembl_rmsk_TE.gtf. Will it still work well in different reference version?

Thanks, Song

olivertam commented 6 months ago

Hi Song,

Thank you for your interest in the software.

I'm assuming you're using GENCODE FASTA for mapping, and the GENCODE v25 GTF for gene annotation. I would recommend using GRCh38_GENCODE_rmsk_TE.gtf, which uses the same chromosome nomenclature as GENCODE.

Unfortunately, a mismatch in chromosome nomenclature will result in no annotation for those features, so it's highly recommended to use annotations and FASTA with the same chromosome names.

Thanks.

songlyzz commented 6 months ago

Thank you very much!

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