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mikemc / vivo-vitro

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Investigate v2_A2_ASV60 #23

Open mikemc opened 3 years ago

mikemc commented 3 years ago

DADA2/Silva assigned this as Mitochondria; yet it Blasts as an exact hit to Bacteroides ovatus 16S; but isn't at all similar to the B. ovatus 16S ASVs I identified in my strain assignment procedure.

mikemc commented 3 years ago

Turns out this ASV is the reverse complement of the primary B. ovatus ASV v2_A2_ASV1. It's frequency is always very small, never going much above 3e-4 and completely absent in most samples, so perhaps this is just from a rare PCR event or something and can be safely ignored.

This does suggest a possibly-useful ASV filtering step of looking for ASVs that are reverse complements of other ASVs