I have been trying to assemble a chloroplast genome with pacbio-hifi data（flye --pacbio-hifi data/qw2010.fasta --out-dir ./out_pacbio --threads 4）. but,there a warning message : No overlaps found - unable to estimate parameters on the terminal. Does that means my data does not meet the input data requirements. or is it due to other reasons?
I have been trying to assemble a chloroplast genome with pacbio-hifi data（flye --pacbio-hifi data/qw2010.fasta --out-dir ./out_pacbio --threads 4）. but,there a warning message : No overlaps found - unable to estimate parameters on the terminal. Does that means my data does not meet the input data requirements. or is it due to other reasons?
here is my logfile: flye.log
You're help would be much appreciated.