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mikolmogorov / Flye

De novo assembler for single molecule sequencing reads using repeat graphs
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Metagenome #681

Closed joao1980 closed 3 months ago

joao1980 commented 4 months ago

Hi, My apologies if this is a basic question but it is the first time I am dealing with Nanopore sequencing data. I plan to process metagenomes from GridION sequencing. I was reading you can use the meta option without the need to estimate genome size and only use asm-coverage. Should this be set to 40x? If I have plenty of computational resources can I just disregard setting the asm-coverage and genome-size options? Thanks for the help!

mikolmogorov commented 3 months ago

Yes, you don't need to set --asm-coverage anymore unless you want to downsample.