Usage of tool for other experimental data

mikpom / uslcount

Quantitation of gene expression from read alignments

GNU General Public License v3.0

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Usage of tool for other experimental data #3

Open Hemantcnaik opened 3 years ago

Hemantcnaik commented 3 years ago

Hello, great tool

I have question regarding usage of the tool, i have RNA seq data unstranded libr prep method, can i use this tool for getting true gene expression

mikpom commented 3 years ago

The tool can give you an improvement for some genes which expression estmation is obscured by opposite strand expression in the same locus. It uses known splice junctions for that. That's the specific occasion the tool might be more useful than more conventional methods.