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milaboratory / mixcr

MiXCR is an ultimate software platform for analysis of Next-Generation Sequencing (NGS) data for immune profiling.
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takara-mouse-rna-bcr-smarter #1587

Closed pbpayal closed 6 months ago

pbpayal commented 6 months ago

Checklist before submitting the issue:

I am running mixcr/4.3.2. I want to run the "takara-mouse-rna-bcr-smarter" preset because the library was prepared using SMARTer Mouse BCR IgG H/K/L Profiling Kit. But I am getting error.

ERROR

It says this preset doesn't exist. *IMPORTANT: MiXCR will use at most 12000MB of RAM, use -Xmx to change automatically set heap size (i.e. -Xmx50g to set heap size to 50 Gb) No preset with name "takara-mouse-rna-bcr-smarter". Here are supported presets with similar names:

Exact MiXCR commands

mixcr analyze takara-mouse-rna-bcr-smarter \ input_R1.fastq.gz \ input_R2.fastq.gz \ result

What preset am I supposed to use for this kit then?

mizraelson commented 6 months ago

Hi, Please update to the latest MiXCR V4.6. The command should work with it.

pbpayal commented 6 months ago

Problem is this package is on a server not managed by me. Let me see.

mizraelson commented 6 months ago

You can use takara-mouse-bcr-full-length in that case, but I would strongly recommend upgrading.