Closed ChoiJi-Hye closed 1 month ago
The command seems correct. It appears that the issue is with the data itself. Could you please share the pair of raw FASTQ files? I can then review them and provide some insights. You can reach us at support@milaboratories.com
Hi! Thanks for creating a great tool!
Expected Result
I used [TakaRa] SMART-Seq Human TCR (with UMIs) to generate bulk TCR data and used your tool for analysis, but most of the results came out as "region_not_covered." When I look at other people's results, most of them seem to have sequences written. Is there an option I might have missed?
I proceeded with the analysis using takara-human-rna-tcr-umi-smartseq, but is this an accurate result? Are there any other issues?
Actual Result
Exact MiXCR commands
Mixcr_4.6.0/mixcr analyze takara-human-rna-tcr-umi-smartseq R_23_00645_FN_ETC_RNA_R1.fastq.gz R_23_00645_FN_ETC_RNA_R2.fastq.gz result_directory/R_23_00645 -t 16
MiXCR report files
Thank you for your help! Ji-Hye Choi