Closed michael-ford closed 3 weeks ago
Hi, because for this kit the reverse primer is located in FR4
region, the clones are assembled by {FR1Begin:CDR3End}
feature to exclude the primer sequence. If needed you can manually add the following parameter to mixcr analyze
command and then findAlleles
would work:
mixcr analyze invivoscribe-human-dna-ighv-leader-lymphotrack \
--assemble-clonotypes-by VDJRegion \
input_R1.fastq.gz \
input_R2.fastq.gz \
output
Checklist before submitting the issue:
Expected Result
Have run
mixcr analyze invivoscribe-human-dna-ighv-leader-lymphotrack
, now trying to runfindAlleles
to call alleles / novel variants. Getting a Feature for allele search doesn't intersect JGene error?Confirmed the following:
mixcr exportAirr
- all clones seem to have V, D and J calls. Example:exportAlignmentsPretty shows reads covering L1 through FR4:
Actual Result
Exact MiXCR commands