Support for BD Rhapsody fulll-length VDJ

[hongduosun]

Thanks for developing such a great tool!

Is there any plan to add support for the BD Rhapsody full-length protocol?

https://www.bdbiosciences.com/en-us/products/reagents/single-cell-multiomics/vdj#Overview

R1 structure:

[mizraelson]

Hi, Yes, we surely will add a full-length protocol support. I haven't found any published data yet to test a full-length preset. If by any chance there is an open access dataset that you know of - please let me know. When the data is available it only will take me a few days to add this functionality. Also, we are currently working on supporting sample tags for BD Rhapsody protocols.

[hongduosun]

Yes, these is a demo dataset: https://scomix.bd.com/hc/en-us/articles/4424059302157-Rhapsody-TCR-BCR-Full-Length-Demo-Dataset Corresonding fastqs: http://bd-rhapsody-public.s3-website-us-east-1.amazonaws.com/Rhapsody-Demo-Data-Inputs/RhapTCRBCRdemo/

[iommuno]

Hi, Thank you for developing and maintaining this cool tool! Just wanted to check in: Is there any update on adding the preset for 5' barcoded TCR/BCR libraries on enhanced beads?

[mizraelson]

Hi, yes, we are preparing the preset for full-length BD rhapsody protocol. It should be ready by the end of the week.

[iommuno]

Awesome, looking forward to trying it! Thanks for getting back to me so quickly.

[PoslavskySV]

Added in v4.2.