using different programs initially

[bioforkgithub]

I want to know if we can use

1. Trimgalore instead of tRIMMOMATIC
2. MEGAHIT INSTEAD OF IDBA
3. And then Prodial Will the program work on a PC to do the work?

[bioforkgithub]

(metacompare) [a40540@styx MetaCompare]$ python3 metacmp.py -c S1.fa -g S1_genes.fa check file path: /gpfs/gpfs0/prosjekt/.prosjekt2/15719-Res-Marine/softwares/MetaCompare Running blastn on ACLAME blastn: error while loading shared libraries: libnsl.so.1: cannot open shared object file: No such file or directory Running blastx on CARD blastx: error while loading shared libraries: libnsl.so.1: cannot open shared object file: No such file or directory Running blastn on PATRIC blastn: error while loading shared libraries: libnsl.so.1: cannot open shared object file: No such file or directory Reading files.. Computing resistome risk score.. Traceback (most recent call last): File "/gpfs/gpfs0/prosjekt/.prosjekt2/15719-Res-Marine/softwares/MetaCompare/metacmp.py", line 98, in if not os.path.getsize(os.getcwd()+"/"+acc_name) > 0: File "/home/a40540/anaconda3/envs/metacompare/lib/python3.10/genericpath.py", line 50, in getsize return os.stat(filename).st_size FileNotFoundError: [Errno 2] No such file or directory: '/gpfs/gpfs0/prosjekt/.prosjekt2/15719-Res-Marine/softwares/MetaCompare/S1_ACLAME.txt'

This is the run that I tried with the test files provided on the github page. It gives an error

[bioforkgithub]

Tried everything. It works on assembly from megahit and trimgalore and prodigal. There was a problem with anaconda blast files. Binaries from NCBI solved the problem.

[shaodongyan]

@bioforkgithub Hello! I hope this message finds you well. I am writing to kindly request your assistance. I am very interested in accessing the database of MetaCompare software, but unfortunately, I do not currently have access to it. I understand that you may have the privilege of accessing or owning this database, and I was wondering if you would be willing to share a copy with me. Your generosity in granting me access would be greatly appreciated, and I would be sincerely grateful for your help.

If there is any additional information you require or if you have any questions, please feel free to let me know. Thank you very much for your time and assistance.

Warmest regards,Dongyan