mitoNGS / MToolBox

A bioinformatics pipeline to analyze mtDNA from NGS data
http://sourceforge.net/projects/mtoolbox/?source=navbar
GNU General Public License v3.0
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.py files "command not found" #110

Open SamiaPT opened 1 year ago

SamiaPT commented 1 year ago

all the .py files are present in the directory and are executable yet while running the command it says "no command found" please help me user@user:~/Desktop/MToolBox-master/MToolBox$ ./MToolBox.sh -i test_rCRS_config.sh

setup.sh file not found. Setting MToolBox environment sourcing conf.sh file

setting up MToolBox variables in config file ... ...done

/home/user/Desktop/MToolBox-master/MToolBox/vcf will be used as vcf file name...

Check python version... (2.7 required) OK.

Checking files to be used in MToolBox execution...

Checking mapExome parameters... OK.

Checking assembleMTgenome parameters... OK.

Checking mt-classifier parameters... OK.

Input type is fastq. output files will be placed in /home/user/Desktop/MToolBox-master/MToolBox/test_out/

EXECUTING READ MAPPING WITH MAPEXOME...

mapExome for sample PD11, files found: PD11.R1.fastq PD11.R2.fastq ./MToolBox.sh: line 250: mapExome.py: command not found mapExome for sample PM11, files found: PM11.R1.fastq PM11.R2.fastq ./MToolBox.sh: line 250: mapExome.py: command not found

SAM files post-processing...

SORTING OUT.sam FILES WITH PICARDTOOLS...

ls: cannot access 'OUT_*': No such file or directory Success.

ls: cannot access 'OUT*': No such file or directory Skip Indel Realigner... ls: cannot access 'OUT*': No such file or directory

ELIMINATING PCR DUPLICATES WITH PICARDTOOLS MARKDUPLICATES...

ls: cannot access 'OUT*': No such file or directory ls: cannot access 'OUT': No such file or directory ls: cannot access 'OUT_': No such file or directory

ASSEMBLING MT GENOMES WITH ASSEMBLEMTGENOME...

WARNING: values of tail < 5 are deprecated and will be replaced with 5

ls: cannot access 'OUT_*': No such file or directory

GENERATING VCF OUTPUT...

Traceback (most recent call last): File "/home/user/Desktop/MToolBox-master/MToolBox/VCFoutput.py", line 4, in from mtVariantCaller import VCFoutput File "/home/user/Desktop/MToolBox-master/MToolBox/mtVariantCaller.py", line 13, in import vcf File "/home/user/Desktop/MToolBox-master/MToolBox/vcf/init.py", line 175, in from vcf.parser import Reader, Writer File "/home/user/Desktop/MToolBox-master/MToolBox/vcf/parser.py", line 4, in import gzip File "/usr/local/lib/python2.7/gzip.py", line 9, in import zlib ImportError: No module named zlib

PREDICTING HAPLOGROUPS AND ANNOTATING/PRIORITIZING VARIANTS...

Haplogroup predictions based on RSRS Phylotree build 17 ./MToolBox.sh: line 479: mt-classifier.py: command not found ./MToolBox.sh: line 483: variants_functional_annotation.py: command not found ./MToolBox.sh: line 484: variants_functional_annotation.py: command not found No annotation.csv found. Exit

user@user:~/Desktop/MToolBox-master/MToolBox$